Research Article
A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Table 4
Reproducibility of operators and experiments.
| | Operator | RNA | Experiment 1 | Experiment 2 | Experiment 3 | Reproducibility of experiments |
| | Operator 1 | Kasumi-1 | AML1-ETO | AML1-ETO | AML1-ETO | | | | THP-1 | MLL-AF9 | MLL-AF9 | MLL-AF9 | 100% | | | REH | TEL-AML1ex2 | TEL-AML1ex2 | TEL-AML1ex2 | | | | HL-60 | None | None | None | | |
| | Operator 2 | Kasumi-1 | AML1-ETO | AML1-ETO | AML1-ETO | | | | THP-1 | MLL-AF9 | MLL-AF9 | MLL-AF9 | 100% | 100% | | REH | TEL-AML1ex2 | TEL-AML1ex2 | TEL-AML1ex2 | | | | HL-60 | None | None | None | | |
| | Operator 3 | Kasumi-1 | AML1-ETO | AML1-ETO | AML1-ETO | | | | THP-1 | MLL-AF9 | MLL-AF9 | MLL-AF9 | 100% | | | REH | TEL-AML1ex2 | TEL-AML1ex2 | TEL-AML1ex2 | | | | HL-60 | None | None | None | | |
| | Reproducibility of operators | | 100% | 100% | 100% | | | | | | 100% | | | |
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