Angiopoietin-Like 3 Induces Podocyte F-Actin Rearrangement through Integrin 𝜶_𝑉𝜷₃/FAK/PI3K Pathway-Mediated Rac1 Activation

Figure 3

Blocking integrin 𝛼_𝑉𝛽₃ blocked actin filament rearrangement and Rac1 and RhoA activation as induced by Angptl3. (a) F-actin in podocytes preincubated with 0.1 μM echistatin α1 isoform for 1 h, followed by treatment with 250 ng/mL Angptl3 for 15 min (B), 30 min (C), or no treatment (A). Scale bar, 20 μm. The comparison of lamellipodia formation between groups is shown in (D). (b) Comparison of RhoA activation between podocytes pretreated with echistatin or left untreated. Compared to control group, *𝑃<0.05, **𝑃<0.01. (c) Comparison of Rac1 activation between podocytes pretreated with echistatin or left untreated. Compared to control group, *𝑃<0.05, **𝑃<0.01.

(a)

(b)

(c)