Association Study of Single Nucleotide Polymorphisms in XRCC1 Gene with Risk of Hepatocellular Carcinoma in Chinese Han Population

Table 2

Primers, PCR-RFLP, and CRS-PCR analyses for genotyping XRCC1 gene polymorphisms.

SNPs	Primer sequences	Annealing temperature (°C)	Amplification fragment (bp)	Region	Restriction enzyme	Genotype (bp)
c.1254C>T	5′-GAGGAGGATGAGGCCTCTCACAC-3′; 5′-TAAGGAGGGAGAGTGGGTGGGT-3′	63.9	218	Exon11	HpaII	CC: 195, 23; CT: 218, 195, 23; TT: 218
c.1517G>C	5′-CAAGTCCCAGCTGAGAACTGAG-3′; 5′-GCTGCTCTGCATGCTCACTC-3′	59.0	247	Exon14	HaeIII	GG: 247; GC: 247, 168, 79; CC: 168, 79

Note: PCR means polymerase chain reaction; PCR-RFLP means PCR-restriction fragment length polymorphism; CRS-PCR means created restriction site PCR; SNPs mean single nucleotide polymorphisms. Underlined nucleotides mark nucleotide mismatches enabling the use of the selected restriction enzymes for discriminating sequence variations.