139745.fig.001a
(a)
139745.fig.001b
(b)
139745.fig.001c
(c)
139745.fig.001d
(d)
Figure 1: 2-DE and Western blot analysis of T. spiralis muscle larval excretory-secretory (ES) proteins. (a) 2-DE gel of ES proteins separated in the first dimension in the pH range 3–10 and then in the second dimension on a 12% polyacrylamide gel. The gel was stained with Coomassie blue R-250, molecular weight standard is on the left, and pI values are indicated. Protein spots selected for analysis are numbered. (b) 2-DE Western blot of ES proteins probed by mouse infection sera at 18 days postinfection (DPI), and the immunoreactive protein spots were detected by the enhanced chemiluminescent (ECL) kit. (c) 2-DE Western blot of ES proteins probed by mouse infection sera at 42 DPI. (d) Western blot map probed by sera from mice before infection.