Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig
Figure 6
Morphologies and protein expression patterns of porcine iPSCs cultured in various conditions. The A15 line is cultured in medium containing (A) 20 ng/mL LIF, (B) 20 ng/mL bFGF, (C) 20 ng/mL BMP-4, (D) 20 ng/mL LIF and + 2 μM SU, or (E) 20 ng/mL bFGF and 1 μM JAKi, for 48 h. ((a)–(e)) All colonies are positive for AP, regardless of their culture conditions. (F) Western blot shows the phosphorylation levels of Stat3 and Smad1/5/8 proteins in A15 line cultured with different conditions, which were quantitated using ImageJ program ((G), (H)). .
