Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig

Table 1

Primer sets for RT- and real-time qPCR.

Gene name	Primer Sets (5′→3′)		References
	Forward	Reverse
For RT-PCR*
hPOU5F1	GATCAAGCAGCGACTATGCA	TCTGGGATGGAAACTGGAA	Ezashi et al. 2009 [5]
hKLF4	CTGCGGCAAAACCTACACAA		Ezashi et al. 2009 [5]
hNANOG	CCACTAGGTATTTTAGTACTCC		NM_024865
hSOX2	CCTGGCATGGCTCTTGGC		Ezashi et al. 2009 [5]
hC-MYC	GATTCTCTGCTCTCCTCGACG		Ezashi et al. 2009 [5]
hLIN28	GCGGCCAAAAGGAAAGAGCA		NM_024674
Gapdh	CTCAACGACCACTTCGTCAA	TCTGGGATGGAAACTGGAAG	X94251
pPou5f1	ACAAGGAGAAGCTGGAGCCG	CGCGGACCACATCCTTCTCT	NM001113060
pKlf4	TGGGCAAGTTTGTGTTGAAG	AGGAAGGGTGGGTAGTTTGG	DQ000310.1
pNanog	TGAGGTTTATGGGCCTGAAG	ATTTCATTCGCTGGTTCTGG	NM_001129971.1
pSox2	CAAGATGCACAACTCGGAGA	TGCTGTAGCTGCAGTTGCTC	NM_001123197.1
pc-Myc	CAGATCAGCAACAACCGAAA	TCCAACTCTGGGATCTGGTC	FJ882404.1
pLin28	TGCACCAGAGTAAGCTGCAC	CTGCATATTCTTCCCCTTGG	HM347046.1
For RT-qPCR
Pou5f1	AGCGCTTCAGAAAGATCTCG	GAGCTGCAAAGCCTCAAAAC	NM001113060
Nanog	GATTGGGGTGGTTAGCTCCT	TGAAGGTGAGACTCGCTCTG	NM_001129971.1
Sox2	CAGGAGGGAAGACTCCATCA	CTCCCTCTTGGACAGTCGAG	NM_001123197.1
Klf4	GCCCTTAGAGGCCCACTT	GCAGGGCAGGATGACAGT	DQ000310.1
Rex1	AGCTAACCCTGTCCACATCG	CAAGTCAGCAGCAGTCTCCA	XM_003123016.2
Socs3	CTCCGACTGAACCCTCCTC	CGTTGACTGTTTTCCGACAG	HM045422.1

∗The reverse primer used for exogenous human POU5F1, KLF4, NANOG, SOX2, C-MYC, and LIN28 expression is a part of woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) region within viPS lentiviral vector. h: human; p: pig; F: forward; R: reverse.