In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Figure 1
Characterization of spermatogonial stem cells (SSCs). (a) Expression of pluripotent stem cells marker (SSEA-4) in the hESCs and testis. (b) Expression of pluripotent stem cells (SSEA-4) and SSCs marker (GFR α1) in the primary seeding cells. The yellow arrows indicate a GFR α1-positive signals which were expressed in SSCs. (c) Localization of specific markers for SSCs (CD9, red, and GFR α1, green) in the cultured cell clumps (at passage 1). (d) Karyotyping of SSCs performed at passage 5. Note: Mock 1st Ab; cultured cells were stained with secondary antibody only as a negative control. Scale bars = 100 μm.
