In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Figure 3
Identification of pluripotent stem cells within spermatogonial stem cell (SSC) clumps using flow cytometry and RT-PCR. (a) Flow cytometric analysis of the cultured SSC clumps using a pluripotent stem cell marker (SSEA-4). (b, c) Immunocytochemical analysis and RT-PCR analysis of pluripotent stem cell markers (OCT-4, NANOG, and Integrin α6) in the cultured SSC clumps. 18S ribosomal RNA was used as an experimental control.
