In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Figure 4
Spontaneous differentiation of spermatogonial stem cell (SSC) clumps in vitro. (a) Morphology of differentiated EB-like structured cells after plating onto non-coated dishes, and hematoxylin and eosin staining of EB-like structured cells. (b) Expression of three germ layer-specific markers in EB-like structured cells derived from SSC clumps. Markers of ectodermal progenitor cells (nestin), endodermal lineage cells (α-fetoprotein) and mesodermal cells (cardiac protein). Note: Mock 1st Ab; cultured cells stained with secondary antibody only as a negative control. Scale bars = 100 μm.
