In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Figure 5
Teratoma formation from transplanted pluripotent stem cells within spermatogonial stem cells (SSCs) (a) Teratoma obtained from hESCs (CHA-hES4) and multipotent stem cells among SSCs. (b) Morphology of three germ layer-like structures obtained from multipotent stem cells within SSCs. (c) Immunohistochemical studies for human specific antibody. α-fetoprotein (ectoderm marker reacts with human), MAP-2 (mesoderm marker reacts with human) and STEM121 (cytoplasm marker reacts with human). Note: Mock 1st Ab; cultured cells stained with secondary antibody only as a negative control. Scale bars = 100 μm.
