Lithium Chloride Enhances Cathepsin H Expression and BMP-4 Degradation in C3H10T1/2 Cells

Figure 4

Gene silencing of cathepsin H was carried out by electroporatic gene transfer of small interfering RNA. siCTSH targeting cathepsin H or siControl (300 pmol/cuvette each) was transfected with pCAGGS-BMP4 (30 μg/cuvette). (a) Quantitative RT-PCR at day 3. Cathepsin H mRNA expression was inhibited significantly. (b) Western blot analysis for BMP4 in the culture medium at day 3 showed marked increase of BMP4. (c) Alcian blue staining of micromass showed significant increase by gene silencing of cathepsin H. ^∗𝑃<0.05, ^∗∗∗𝑃<0.001.

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