About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 146079, 11 pages
http://dx.doi.org/10.1155/2013/146079
Research Article

The Association of the Immune Response Genes to Human Papillomavirus-Related Cervical Disease in a Brazilian Population

1Campinas State University, Rua Carlos Chagas 480, Barão Geraldo, 13083878 Campinas, SP, Brazil
2Basic Health Sciences Department, Maringa State University, Avenue Colombo 5790, 87020900 Maringá, PR, Brazil
3Pathology Sciences Department, Londrina State University, Rodovia Celso Garcia Cid (PR 445), 86051-970 Londrina, PR, Brazil
4Department of Clinical Analysis and Biomedicine, Maringa State University, Avenue Colombo 5790, 87020900 Maringá, PR, Brazil
5Program of Biosciences Applied to Pharmacy, Department of Clinical Analysis and Biomedicine, Maringa State University, Avenue Colombo 5790, 87020900 Maringá, PR, Brazil

Received 29 March 2013; Revised 5 June 2013; Accepted 15 June 2013

Academic Editor: Enrique Medina-Acosta

Copyright © 2013 Amanda Vansan Marangon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genetic variability of the host contributes to the risk of human papillomavirus (HPV)-related cervical disease. Immune response genes to HPV must be investigated to define patients with the highest risk of developing malignant disease. The aim of this study was to investigate the association of polymorphic immune response genes, namely KIR, HLA class I and II, and single-nucleotide polymorphisms (SNPs) of cytokines with HPV-related cervical disease. We selected 79 non-related, admixed Brazilian women from the state of Paraná, southern region of Brazil, who were infected with high carcinogenic risk HPV and present cervical intraepithelial neoplasia grade 3 (CIN3), and 150 HPV-negative women from the same region matched for ethnicity. KIR genes were genotyped using an in-house PCR-SSP. HLA alleles were typed using a reverse sequence-specific oligonucleotide technique. SNPs of TNF −308G>A, IL6 −174G>C, IFNG +874T>A, TGFB1 +869T>C +915G>C, and IL10 −592C>A −819C>T −1082G>A were evaluated using PCR-SSP. The KIR genes were not associated with HPV, although some pairs of i(inhibitory)KIR-ligands occurred more frequently in patients, supporting a role for NK in detrimental chronic inflammatory and carcinogenesis. Some HLA haplotypes were associated with HPV. The associations of INFG and IL10 SNPs potentially reflect impaired or invalid responses in advanced lesions.