Skeletal Muscle Regeneration on Protein-Grafted and Microchannel-Patterned Scaffold for Hypopharyngeal Tissue Engineering
Figure 7
Immunofluorescence staining. Cells were immunostained with primary anti-MyoD1 antibody and anti-mouse IgG (H+L) secondary antibody conjugated with FITC (green, 2). Cell nuclei were stained with DAPI (blue, 1). (3) is the composite of (1) and (2). Cells were seeded at the density of 8 × 104 cells mL−1 and cultured for 10 days on three different scaffolds, PU (a): PU-Gel (b), and PU-SF (c).
