Figure 2: Single cell migration in MCF-7 and T47D cells after their treatment with E2 and antiestrogens. C: control (untreated cells); E2: cells treated with 17β-estradiol; Fulv: cells treated with E2 + 100 nM Fulv; Tam: cells treated with E2 + 100 nM Tam; End: cells treated with E2 + 100 nM End; and 4-OH-T: cells treated with E2 + 100 nM 4-OH-T. The image is representative of three independent experiments using a magnification of 10X (a). Quantification of images from boyden chamber assay in MCF-7 cells (b). Results are expressed as mean ± SEM of the % change compared to the untreated cells. Asterisks denote a statistically significant difference compared to E2 treated cells. ** and ***.