A Recombinant Multiepitope Protein for Hepatitis B Diagnosis
Figure 2
Time course rMEHB expression in E. coli BL21 (λDE3) and analysis by SDS-PAGE12%. (a) Lane M, unstained protein molecular mass marker (Fermentas Life Sciences); Lane 1, uninduced control; Lanes 2–4, after 0.5, 1.5, and 2.5 hours of induction, respectively. (b) Western blotting analysis of the purified rMEHB. The monoclonal antibody, antipolyhistidine conjugated alkaline phosphatase, was diluted 1 : 1000 in PBS.