Research Article

A Recombinant Multiepitope Protein for Hepatitis B Diagnosis

Figure 6

Enzyme linked immuno sorbent assay (ELISA) using rMEHB and monoclonal antibody Anti-HB, present on the plate surface of a commercial kit for hepatitis B diagnosis. Several concentrations of rMEHB were used as following: 50 ng, 500 ng, 1 μg, 10 μg, and 15 μg. PBS was used as negative control. Antipolyhistidine conjugated alkaline phosphatase was the second antibody and the substrate was pNPP.
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