A Recombinant Multiepitope Protein for Hepatitis B Diagnosis

Table 2

Secondary structure content of rMEHB calculated from far-UV (260–195 nm) CD curves adjustments in 2 mM MOPS pH 7.0 and 8.0 using the network secondary structure estimator from concentration independent Raussens et al. method (http://perry.freeshell.org/raussens.html) [25].

Secondary structure (%)	pH 7.0		pH 8.0
	25°C	95°C	25°C	95°C
α-helix	7.5	5.3	8.0	7.4
β-sheet	32.7	36.7	33.1	33.1
β-turn	12.5	14.5	12.5	12.9
Random-coil	40.9	40.5	40.8	40.9