Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets

Figure 2

Evaluation of islet viability. (a) AO-PI double fluorescent staining was performed to visualize cell viability. Viable islets are green and dead islets are red (×200) (bar = 100 µm). (b) Islets were cultured and their viability was assessed using a CCK-8 assay. The optical density (OD) at 450 nm was determined at the indicated time points as a measure of cell viability. Significant increases are indicated by “†” at 𝑃<0.05, “++” at 𝑃<0.01, and “+++” at 𝑃<0.001, compared with the static group. A significant difference between PGA-sMG and PGA or sMG is denoted by “#.”

(a)

(b)