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BioMed Research International
Volume 2013 (2013), Article ID 150739, 7 pages
Simulated Microgravity Combined with Polyglycolic Acid Scaffold Culture Conditions Improves the Function of Pancreatic Islets
1Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China
2The Key Laboratory of Cell Transplantation of Ministry of Health and Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Nangang District, Harbin, Heilongjiang, China
3General Surgery, Heilongjiang Provincial Hospital, Nangang Branch, Harbin 150001, China
Received 28 February 2013; Revised 1 June 2013; Accepted 16 June 2013
Academic Editor: Manoor Prakash Hande
Copyright © 2013 Yimin Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
FIGURE S1: Evaluation of islet viability in long-term culture. Among the four conditions, the static group demonstrated the worst survival rate in the long culture. The cells cultured in the sMG condition displayed a better effect on preserving islets survival than those in PGA. Comparably, the PGA-sMG treatment was proven to have a preferable ability to maintain the cell viability as compared with any one of the other three groups. Significant increases between PGA and sMG and between PGA-sMG and PGA or sMG are denoted by “#”.
FIGURE S2: Assessment of the functionality of insulin production of cells in long-term culture. Compared with the other three groups, the static condition undoubtedly failed to reverse decreased insulin secretion. The sMG group had a preferable role in improving the secretory function of islets than the PGA group. The cells in the PGA-sMG condition displayed the strongest ability to promote insulin production across all studied conditions. Significant increases between PGA and sMG and between PGA-sMG and PGA or sMG are denoted by “#”.
FIGURE S3: Observable biodegradability of PGA scaffolds at days 25 in PGA-sMG culture condition under scanning electron microscopy (×1800) (bar = 50 µm). The degraded fragments of the scaffolds were visualized in the PGA-sMG group under SEM.