The Effect of Msh2 Knockdown on Toxicity Induced by tert-Butyl-hydroperoxide, Potassium Bromate, and Hydrogen Peroxide in Base Excision Repair Proficient and Deficient Cells

Figure 2

MTT and clonogenic survival curves for t-BOOH treatment of Nth1 ^+/+ and Nth1 ^−/− MEFs with and without reduced Msh2 expression. MTT (a) and clonogenic survival (b) curves for Nth1 ^+/+ + pshRNAMsh2283, clone 2 (88% Msh2 gene silencing; black square) and Nth1 ^+/+ + pshRNA empty (grey square) treated with t-BOOH. MTT (c) and clonogenic survival (d) curves for Nth1 ^−/− + pshRNAMsh2283, clone 1 (85% Msh2 gene silencing; solid square), or Nth1 ^−/− + pshRNA empty (grey square) treated with t-BOOH. Results are expressed as mean ± SEM; ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001.

(a) Nth1 ^+/+ + t-BOOH

(b) Nth1 ^+/+ + t-BOOH

(c) Nth1 ^−/− + t-BOOH

(d) Nth1 ^−/− + t-BOOH