The Effect of Msh2 Knockdown on Toxicity Induced by tert-Butyl-hydroperoxide, Potassium Bromate, and Hydrogen Peroxide in Base Excision Repair Proficient and Deficient Cells

Figure 5

MTT and clonogenic survival curves for KBrO₃ treatment of Mpg ^+/+ and Mpg ^−/− MEFs with and without reduced Msh2 expression. MTT (a) and clonogenic survival (b) curves for Mpg ^+/+ + pshRNAMsh2283, clone 9 (85% Msh2 gene silencing; black square), or Mpg ^+/+ + pshRNA empty (grey square) cells treated with KBrO₃. MTT (c) and clonogenic survival (d) curves for Mpg ^−/− + pshRNAMsh2283, clone 1 (79% Msh2 gene silencing; solid square), or Mpg ^−/− + pshRNA empty (grey square) cells treated with KBrO₃. Results are expressed as mean ± SEM; *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001.

(a) Mpg ^+/+ + KBrO₃

(b) Mpg ^+/+ + KBrO₃

(c) Mpg ^−/− + KBrO₃

(d) Mpg ^−/− + KBrO₃