About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 153419, 5 pages
Research Article

Bioaccumulation Experiments in Mussels Contaminated with the Food-Borne Pathogen Arcobacter butzleri: Preliminary Data for Risk Assessment

Istituto Zooprofilattico Umbria e Marche, Sezione di Ancona, Laboratorio Nazionale di Riferimento (LNR) Contaminazioni Batteriologiche Molluschi Bivalvi Vivi, Via Cupa di Posatora 3, 60126 Ancona, Italy

Received 23 April 2013; Revised 16 July 2013; Accepted 7 August 2013

Academic Editor: Matthias Labrenz

Copyright © 2013 Donatella Ottaviani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to evaluate, at a laboratory scale, the ability of this microorganism to grow in seawater and bioaccumulate in mussels (Mytilus galloprovincialis) maintained in constantly aerated tanks, containing twenty litres of artificial seawater. Three concentrations of A. butzleri LMG were tested (about  CFU/mL,  CFU/mL, and  CFU/mL). Following contamination, enumeration of A. butzleri was performed from water and mussels each day, for up to 96 h. Three contamination experiments with artificial seawater in absence of mussels were also performed in the same manner. In the experiments with mussels, A. butzleri declined in water of approximately 1 log every 24 h from the contamination. In artificial seawater without mussels the concentration of A. butzleri remained on the same logarithmic level in the first 48 h and then decreased of about 1 log every 24 hours. In mussels, the concentration was approximately 2 log lower than the exposition level after 24 h from the contamination, and then it decreased exponentially of 1 log every 24 h. Our findings suggest that in the experimental conditions tested A. butzleri is neither able to effectively grow in seawater nor bioaccumulate in mussels, at least in the free and cultivable form.