|(1) Biogenesis Screen (BGS)||This screen studied the biogenesis of the small ribosomal subunit in human cells (40S subunit). Towards this, an assay for visual detection of nuclear maturation defects of the 40S subunit was developed. In the cellular process, a HeLa cell line bearing a fluorescently tagged ribosomal protein of the 40S subunit (Rps2-YFP), which is expressed only upon induction, was used to selectively visualize newly synthesized 40S subunits. This assay has been developed to detect nuclear 40S maturation defects upon depletion of a protein by RNAi. This allowed to identify proteins functioning in 40S biogenesis in human cells and, according to the classification, assign their requirement to nucleolar or nucleoplasmic maturation events. |
Screening results data for QC: 76747 data points corresponding to individual siRNA oligonucleotide (4 siRNAs per targeted gene), including 4 numeric parameters and gene/siRNA annotation.