Simultaneous Quantification of Mitochondrial DNA Damage and Copy Number in Circulating Blood: A Sensitive Approach to Systemic Oxidative Stress
Figure 4
Ex vivo mtDNA damage response to exogenous H2O2 in isolated lymphocytes and whole blood. Experiments were performed to detect the baseline damage level, induced damage by H2O2 treatment, and repair capacity of white blood cells from healthy volunteers. Lymphocytes were isolated from fresh blood of three volunteers and stored at −80°C. The lymphocytes were split into three groups: untreated control (), 15 min of exposure to 120 μM H2O2 (), and 15 min of exposure + 60 min recovery (). (a) Total mtDNA content of lymphocytes. (b) MtDNA damage response of lymphocyte samples. (***).
