Simultaneous Quantification of Mitochondrial DNA Damage and Copy Number in Circulating Blood: A Sensitive Approach to Systemic Oxidative Stress

Table 1

Primer sequences.

Primers	Forward 5′–3′	Reverse 5′–3
CO2 3285 bp long fragment	CCTAGGGTTTATCGTGTGAG	CTAGTTAATTGGAAGTTAACGG
D-loop 2467 bp long fragment	CGCACGGACTACAACCACGAC	CTGTGGGGGGTGTCTTTGGGG
Calicin 2658 bp long fragment	ATTCCAGAAGCCTTTAACTAG	ACAAATGAGACACAAACTACCG
CO2 (for real-time PCR)	CCCCACATTAGGCTTAAAAACAGAT	TATACCCCCGGTCGTGTAGCGGT
D-loop (for real-time PCR)	TATCTTTTGGCGGTATGCACTTTTAACAGT	TGATGAGATTAGTAGTATGGGAGTGG
Calicin (for real-time PCR)	CTGGTCGCTACATCTACATCTC	CAGGTCAGGCAACTTGGTC