Safety Evaluation of Turmeric Polysaccharide Extract: Assessment of Mutagenicity and Acute Oral Toxicity

Table 5

Clastogenicity study upon long term exposure (36 h) of NR-INF-02 in the absence of metabolic activation in human blood lymphocytes.

Treatment	Vehicle control		Positive control MMC; (0.20 µg/mL)		NR-INF-02; (2500.00 µg/mL)		NR-INF-02; (791.14 µg/mL)		NR-INF-02; (250.36 µg/mL)
	R1	R2	R1	R2	R1	R2	R1	R2	R1	R2
Total number of metaphase analyzed	100	100	100	100	100	100	100	100	100	100
Normal	98	99	89	95	97	99	96	100	100	100
Chromatid break	—	—	4	2	—	—	1	—	—	—
Chromosome break	—	—	—	—	—	—	—	—	—	—
Deletion	—	—	4	3	1	—	—	—	—	—
Ring	1	1	1	—	1	1	1	—	—	—
Dicentric	—	—	1	—	—	—	2	—	—	—
Fragment	—	—	—	—	1	—	—	—	—	—
Gap	—	—	—	—	—	—	—	—	—	—
Ploidy	1	—	1	—	—	—	—	—	—	—
Endoreduplication	—	—	—	—	—	—	—	—	—	—
Total aberrations	1	1	10	5	2	1	4	0	0	0
Total aberrations (Mean ± SD)	1.00±0.00		7.50±3.53^∗		1.50±0.70		2.00±2.82		0.00±0.00
Mitotic Index (Mean ± SD)	3.41±0.41		2.15±0.07^∗		2.86±0.23		2.85±0.28		3.01±0.01

R: replicate; ^∗𝑃<0.05; MMC: mitomycin C; Ploidy, endoreduplication, gaps, and fragments are not considered as aberrations for calculations.