Gemifloxacin, a Fluoroquinolone Antimicrobial Drug, Inhibits Migration and Invasion of Human Colon Cancer Cells
Figure 3
The role of Snail in GMF-mediated cell migration and invasion inhibition. (a) The effect of overexpression of Snail in GMF-treated SW620 cells. (b) Snail overexpression did not affect viability of SW620 cells. Snail overexpression decreased the inhibitory effect of GMF on cell migration (c) and invasion (d). Cells were transfected with pCMV or pSnail plasmids, and stable colonies were established by G418 selection. Snail-overexpressing cells were treated with GMF (20 μg/mL) (24 hours for E-cadherin), and various protein levels were assessed by immunoblot assays. Each value is the mean ± SD of three determinations, and all results are representative of at least three independent experiments. The asterisk (*) indicates a significant difference between the two test groups, as analyzed by Student’s t test ().
