Gemifloxacin, a Fluoroquinolone Antimicrobial Drug, Inhibits Migration and Invasion of Human Colon Cancer Cells
Figure 4
GMF inhibited NF-κB activity in SW620 cells. GMF decreased NF-κB nuclear translocation (a) and activity (b) in the SW620 cells. (c) GMF increased I-κBα levels in the cytosol. Cells were treated with various concentrations of GMF. Nuclear and cytoplasm fractions were separated by a nuclear extract kit, and protein expressions were assessed by immunoblot assay. The DNA binding activity of NF-κB in the nuclear fraction was assessed by a Trans-AM ELISA kit. All results are representative of at least three independent experiments. Each value is the mean ± SD of three determinations. The asterisk (*) indicates a significant difference between the two test groups, as analyzed by Student’s t test ().
