Fish Tank Granuloma Caused by Mycobacterium marinum in Two Aquarists: Two Case Reports

Table 2

The presence of Mycobacterium marinum in clinical specimens studied using the erp and IS2404 qPCR assay.

Sample		erp/IS2404  qPCR	ZN microscopy	Cultivation	Isolate identity
Matrice	Source
Patient 1
Subcutaneous nodule	Left knee	1.6×10^7	AFB	+	M. marinum
Cutaneous tissue	Left knee	−	−	−	−
Patient 2
Sperm	Testis	−	−	−
Puncture	Metacarpal joint	2.8×10^5	AFB	+	M. marinum
Pus	Ring finger	5.2×10^6	AFB	+	M. marinum
Blood	Vein	6.2×10^3	−	−	−
Subcutaneous nodule	Right forearm	4.1×10^5	−	+	M. marinum
Tissue	Epididymis	2.8×10^7	AFB	−	−
Tissue	Testis	1.2×10^5	AFB	−	−

Quantification of M.  marinum is shown in genome equivalents per g of tissue or mL of liquid sample.
AFB: acid fast bacilli.