Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions

Figure 2

Time course of the relative expression of CYP11B2 mRNA in NRCMs. NRCMs were grown in culture medium containing either 5.5 mM (gray) or 25 mM (black) glucose for the indicated periods of time (2 hours, 𝑛=6 each; 4 hours, 𝑛=15 each; 8 hours, 𝑛=5 each; 12 hours, 𝑛=6 each; 24 hours, 𝑛=5 each). The qPCR data were normalized to 18S rRNA. The results obtained from cells cultured under normal glucose conditions (gray bars) are shown as the fold changes normalized to the levels after 5.5 mM exposure for four hours, whereas the significance of the differences in the high glucose concentration (black bars) was assessed by comparison of the data with those from the corresponding normal glucose controls (**𝑃<0.01).