Rapid Induction of Aldosterone Synthesis in Cultured Neonatal Rat Cardiomyocytes under High Glucose Conditions

Figure 3

Time course of high glucose-stimulated aldosterone secretion from cardiomyocytes into the culture medium. NRCMs were grown in culture medium containing either 5.5 mM (gray) or 25 mM (black) glucose for the indicated periods of time (4 and 8 hours, 𝑛=14 each). The white bar indicates the aldosterone concentration in the culture medium at baseline after primary myocyte culture, which was followed by an 18-hour serum-free incubation, as described in Section 2 (𝑛=12). The aldosterone concentration secreted into the culture medium was measured using an enzyme immunoassay. **𝑃<0.01 versus baseline, ^#𝑃<0.05 versus 5.5 mM glucose at four hours.