161456.fig.001
Figure 1: pH dependence of the visible spectra of hDDC. Absorbance spectra of 10 μM hDDC were acquired in 50 mM Bis-Tris-propane at pH 6.0, 6.4, 7.0, 7.4, 7.7, 8.0, 8.2, and 8.5. The inset shows the pH dependence of the absorbance at 420 (•) and 335 nm (∘). The solid lines represent the theoretical curves according to (2) and (3).