Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH
Figure 7
Single-wavelength stopped-flow measurements of the reaction of hDDC with L-Dopa at pH 6.0 and 15°C. Reaction of hDDC (20 μM) with L-Dopa (1 mM) was carried out at 15°C in 50 mM Bis-Tris-propane (pH 6.0). Time courses at 420 and 335 nm are reported. The solid lines are from fits to (7). The inset shows the dependence of for the increase in the intensity of the 420 nm band as a function of the final concentrations of L-Dopa after mixing. The points shown are the experimental values, while the curve is from fit to the data using (8).
