In Silico Prediction and In Vitro Characterization of Multifunctional Human RNase3

Figure 2

Sequences of HBR1, HBR2, and HBR3 in hRNase3 and corresponding regions of other 12 hRNaseA members. Sequences of hRNase3 and other hRNaseA superfamily members are aligned using ClustalW2 software. Putative HBR1, HBR2, and HBR3 separately located on residues 34–38, 73–77, and 101–105 are predicted from hRNase3. Residues in black, red, and green boxes indicate corresponding sequence motifs aligned with, respectively, HBR1, HBR2, and HBR3 in all hRNaseA superfamily members.