In Silico Prediction and In Vitro Characterization of Multifunctional Human RNase3

Figure 4

Endocytosis of wild-type and mutant hRNase3 to Beas-2B cells. Endocytosis activity of hRNase3 and its HBR mutants were assayed by western blotting employing 1 : 5000 dilution of anti-His-antibody. Actin was used to normalize the hRNase3-6His immunoblot signal. Forty micrograms of total protein in cell lysates were separated by 15% SDS-PAGE. IB: immunoblot; lane 1: wild-type hRNase3; lane 2-HBR1-mtRNase3; lane 3-HBR2-mtRNase3; lane 4-HBR3-mtRNase3; lane 5-HBR12-mtRNase3; lane 6-HBR13-mtRNase3; lane 7-HBR23-mtRNase3; lane 8-HBR123-mt RNase3; lane 9-cell only.