In Silico Prediction and In Vitro Characterization of Multifunctional Human RNase3
Figure 9
Interaction of hRNase3 and with membrane lipid fractions. Interaction of hRNase3 and FITC- on lipid overlay blot of SpingoStrips membranes (a) and PIP Strips membranes (b). Membranes were incubated with 1 μg/mL hRNase3 or 0.5 μg/mL FITC- at 37°C for 2 h, separately. The immunoreactive blot of hRNase3 treatment was visualized by enhanced chemiluminescence, and the FITC- treatment was using 488 nm laser with standard 530 nm ± 30 nm bandpass emission filter.
