About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 171573, 6 pages
http://dx.doi.org/10.1155/2013/171573
Research Article

Allicin Attenuates Inflammation and Suppresses HLA-B27 Protein Expression in Ankylosing Spondylitis Mice

1Department of Orthopaedics, Shanghai Tenth People’s Hospital Affiliated to TongJi University, Shanghai 20072, China
2Department of Orthopaedics, The Second Affiliated Hospital of the Second Military Medical University, No. 415 Fengyang Road, Huangpu District, Shanghai 200003, China

Received 21 March 2013; Revised 18 September 2013; Accepted 18 September 2013

Academic Editor: Kazim Husain

Copyright © 2013 Xin Gu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Here we aimed to determine the therapeutic effect of allicin on ankylosing spondylitis (AS) and explore the mechanism(s) of action. AS mouse model was constructed by transferring the HLA-B2704 gene into Kunming mice and verified by RT-PCR and CT imaging. Verified AS mice were randomly divided into model group ( ) and allicin-treated groups (50, 100, and 200 mg/kg, resp., , p.o., for 2 months). Wild type mice were used as control ( ). The levels of AS-related inflammatory factors were measured by ELISA. mRNA and protein expressions of HLA-B27 were checked by RT-PCR and western blotting. As the results, the mouse model of AS was successfully established, and high-dose allicin could markedly alleviate spine inflammatory injury possibly via reducing the secretion of the inflammatory factors (IL-6, IL-8, and TNF-α) sharply in AS mice. Moreover, allicin significantly inhibited HLA-B27 protein translation but failed to suppress HLA-B27 gene transcription in AS mice, indicating a posttranscriptional mechanism of this modulation. In conclusion, allicin has potential to be used for AS treatment as an anti-inflammatory nutraceutical.