Table 2: Effect of protease inhibitors, metal ions, and solvents on proteolytic activity of B. amyloliquefaciens MA20.

SubstanceFinal concentrationRelative activity (%)Final concentrationRelative activity (%)

Control01000100
β-mercaptoethanol5 mM181 mM98
PMSF5 mM01 mM0
EDTA5 mM351 mM97
SDS0.5%540.1%122
ZnCl210 mM1535 mM156
MgCl210 mM1195 mM95
CuSO410 mM2665 mM204
Urea10 mM1145 mM139
HgCl210 mM100.45 mM107
CaCl210 mM1045 mM107
BaCl210 mM1155 mM116
Guanidin HCl10 mM1035 mM132
MnCl210 mM715 mM162
Methanol1%900.5%100
Ethanol1%1030.5%100
DMSO1%900.5%103
Isopropanol1%980.5%108
Tween 201%860.5%94
Triton X1001%610.5%65