Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem

Table 3

Effect of protease inhibitors, metal ions, and solvents on proteolytic activity of B. subtilis MA21.

Substance	Final concentration	Relative activity (%)	Final concentration	Relative activity (%)
Control	0	100	0	100
β-mercaptoethanol	5 mM	48	1 mM	70
PMSF	5 mM	0	1 mM	0
EDTA	5 mM	13	1 mM	96
SDS	0.5%	25	0.1%	99
ZnCl2	10 mM	92	5 mM	93
MgCl2	10 mM	22	5 mM	98
CuSO4	10 mM	23	5 mM	73
Urea	10 mM	24	5 mM	52
HgCl2	10 mM	27	5 mM	37
CaCl2	10 mM	65	5 mM	142
BaCl2	10 mM	29	5 mM	69
Guanidin HCl	10 mM	42	5 mM	49
MnCl2	10 mM	64	5 mM	67
Methanol	1%	92	0.5%	99
Ethanol	1%	92	0.5%	97
DMSO	1%	78	0.5%	96
Isopropanol	1%	98	0.5%	101
Tween 20	1%	78	0.5%	86
Triton X100	1%	57	0.5%	133