Research Article
Downregulation of DLC-1 Gene by Promoter Methylation during Primary Colorectal Cancer Progression
Table 1
Primers for MS-HRMA, HRMA, and real-time PCR.
| Usage | Primer | Sequence (5′–3′) | Amplicon (bp) |
| MS-HRMA | MS-HRM-F | TCGTTACGGTTTTAGAAAGAAA | 134 | MS-HRM-R | TTCGCTCCCAACCAAAACATAA |
| HRMA | KRAS-F | CTGAATATAAACTTGTGGTAGTTGGA | 59 | KRAS-R | TATCGTCAAGGCACTCTTGC | BRAF-F | GGTGATTTTGGTCTAGCTACAG | 147 | BRAF-R | AGTAACTCAGCAGCATCTCAGG |
| Real-time PCR | GAPDH-F | GAAGGTGAAGGTCGGAGTCA | 226 | GAPDH-R | GAAGATGGTGATGGGATTTC | DLC-1-F | ACCTGATCACGCAACAGTGAAACA | 191 | DLC-1-R | AGACGCCTGCATAGAGCCTCA |
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