Acute and Subchronic Toxicity Study of Euphorbia hirta L. Methanol Extract in Rats

Table 4

Relative organ weights of rats treated with Euphorbia hirta extract in subchronic toxicity study.

Organs	Control	Euphorbia hirta
		50 mg/kg	250 mg/kg	1000 mg/kg^a	1000 mg/kg^b
Male
Heart	0.44±0.01	0.43±0.02	0.39±0.01	0.41±0.01	0.42±0.01
Liver	2.96±0.08	3.04±0.15	3.02±0.05	2.98±0.07	3.07±0.09
Spleen	0.29±0.01	0.28±0.02	0.27±0.01	0.26±0.02	0.27±0.01
Kidneys	0.72±0.01	0.75±0.01	0.71±0.02	0.73±0.02	0.67±0.03
Lungs	0.90±0.08	0.81±0.07	0.83±0.1	0.72±0.03	0.73±0.04
Female
Heart	0.46±0.02	0.45±0.02	0.45±0.02	0.45±0.01	0.42±0.01
Liver	3.11±0.14	3.35±0.07	3.26±0.02	3.32±0.10	3.27±0.07
Spleen	0.36±0.02	0.32±0.01	0.30±0.02	0.30±0.02	0.30±0.01
Kidneys	0.75±0.02	0.78±0.02	0.75±0.01	0.75±0.04	0.70±0.04
Lungs	0.79±0.04	0.93±0.11	0.88±0.03	0.87±0.05	0.70±0.01

Values are expressed as mean ± SEM (𝑛=10 for each group).
Relative organ weight was calculated as (organ weight/body weight) × 100%.
^aA group was treated with methanol extract of Euphorbia hirta at 1000 mg/kg/day for 90 days.
^bA satellite group was treated with the methanol extract of Euphorbia hirta at 1000 mg/kg/day for 90 days followed by no treatment for 28 days.
^∗𝑃 values < 0.05 were considered significant using one way ANOVA followed by Tukey’s multiple comparison tests. Asterisks denote significant difference compared to control.