Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells
Figure 2
Immunoassayable PAPP-A using antibodies with different specificity. Titration of PAPP-A concentrations from CSp-CM (a) and CDC-CM (b) by three different EIAs. A0230 antibody (recognizing both dPAPP-A and PAPP-A/proMBP), 4PD4 antibody (specific for dPAPP-A), and 5H9 antibody (specific for the proMBP moiety) were used as capture antibodies for both CMs. In all cases, P0042 polyclonal antibody (HRP—conjugated anti-PAPP-A) was used for detection. The anti-proMBP/anti-PAPP-A antibody combination failed to recognize PAPP-A/proMBP complex in both CMs, whereas it was previously shown to be reactive with the pooled third trimester pregnancy serum-derived WHO 78/610 RP and with first and third trimester pregnancy human placental extracts [27].
