Table 1: Primers used for RT-qPCR analysis of chicken mRNAs.

GENEGenBank PRIMERS 5′-3′Tm °CAmplicon bpProbe

EDN1 XM_418943.3gccagccagagagacaagaa606229
tgagcccagagatcttttcc59
TGF-β1(TGF-β4) JQ423909gtccgggctctgtacaaca598717
ccaatactcatcgggtccat59
TGF-β2 NM_001031045ccaggttctgaaatccaaaga59629
ccactttgctgtcaatgtaacg60
TGF-β3 NM_205454gggccctggataccaactac606225
aagaggacgcacacagcag60
TβR I NM_204246ggaaattgctcgtcgatgtt606531
ggtcataatatggcaactggtaatc59
TβR II NM_205428gagttcaagcaccacgacaa59617
cctcggggatcttctcct59
BAMBI NM_001195401acagaggactgcacgatgttt596112
ctgctcccttgtcctgaagt59
ACTB NM_205518gctctgactgaccgcgtta606076
acgagcgcagcaatatcat59
GAPDH NM_204305gtcctctctggcaaagtcca606549
accatgtagttcagatcgatgaag59