The Impact of Hepatic Steatosis on Hepatic Ischemia-Reperfusion Injury in Experimental Studies: A Systematic Review

Table 2

Histological findings in experimental models of hepatic warm ischemia-reperfusion injury and hepatic steatosis.

Author	Animal	Steatosis model	% steatosis	Type of steatosis	Duration of ischemia; (mins, type)	Duration of reperfusion (mins)	Outcome measures	Results in steatotic livers; (lean livers)
Yamada et al. [38]	Rat	D-C	30–60	MiS	30 (partial)	240	HIS	5 (5)^1
Yamada et al. [39]	Rat	D-C	30–60	MiS	30 (partial)	24 hours	HIS	6 (6)^1
Ellett et al. [29]	Mouse	HFD	30–60	MaS	35 (total)	24 hours	HIS	1.4 ± 0.1 (0.4 ± 0.1)^2
Yamagami et al. [30]	Rat^3	CDD	40–60	MaS	45 (total)	40 or 180	Histo	Severe congestion and necrosis
He et al. [31]	Mouse	HFD	50–60	MaS	45 (total)	24 hours	HIS	1.8 ± 0.2 (0.9 ± 0.1)^2
Marsman et al. [40]	Rat	CMDD	>60	MaS	40 (total)	24 hours	Histo (% necrosis)	37 ± 10 (5 ± 1%)^2
Andraus et al. [41]	Rat	PFD	>60	MaS	60 (partial)	240	Histo	↑ Intraparenchymal haemorrhage
Selzner et al. [33]	Mouse	CDD	>60	Mixed	45 or 60 (partial)	24 hours	Histo (% necrosis)	65 ± 24% (25 ± 12%)^2
Caraceni et al. [34]	Rat	CDD	>60	MaS	60	30 to 24 hours	Histo	↑ Sinusoidal congestion and necrosis
Hakamada et al. [35]	Rat	CDD	>60	MaS	30 or 60 (total)	360	Histo	↑ Sinusoidal congestion and necrosis
Hui et al. [36]	Rat	CDD	>70	MaS	30, 45, or 60	60	Histo	Severe necrosis and haemorrhage; (no morphological change)
Takahashi et al. [37]	Canine	HFD	7–99	MaS	60	24 hours	Histo	↑ Sinusoidal congestion and necrosis

CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; D-C: dextrose with cholesterol; HFD: high-fat diet; HIS: histological injury score; Histo: histology; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; PFD: protein-free diet.
1No significant difference between steatotic and lean livers; 2P < 0.05 versus lean livers; 3no lean group in study.