Table 3: Liver function tests in experimental models of hepatic warm ischemia-reperfusion injury and hepatic steatosis.

AuthorAnimalSteatosis model% steatosisType of steatosisDuration of ischemia  
(mins, type)
Duration of reperfusion (mins)Outcome measuresResults in steatotic livers
(lean livers)

Luo et al. [42]MouseHFD + CMDD<30Mixed15 (total)180ALT
AST
450 ± 100 (120 ± 10 IU/L)1  
480 ± 100 (210 ± 20 IU/L)1

Domenicali et al. [43]Rat2CDD>30MaS60 (total)120ALT7500 ± 500 IU/L

Yamada et al. [38]RatD-C30–60MiS30 (partial)240ALT4590 ± 811 (4951 ± 788 IU/L)3

Yamada et al. [39]RatD-C30–60MiS30 (partial)24 hoursALT476 ± 244 (368 ± 97 IU/L)3

Ellett et al. [29]MouseHFD30–60MaS35 (total)24 hoursALT290 ± 120 (130 ± 30 IU/L)

Koti et al. [44]Rat2HC30–60MaS45 (partial)120ALT
AST
5436.3 ± 984.7 IU/L
3166.3 ± 379.6 IU/L

Yamagami et al. [30]Rat2CDD40–60MaS45 (total)40 or 180ALT
AST
518.7 ± 80.3 IU/L
467.7 ± 185 IU/L

He et al. [31]MouseHFD50–60MaS45 (total)24 hoursALT180 ± 10 (80 ± 5 IU/L)1

Fusai et al. [45]Rabbit2HC30–60MaS60 (partial)360ALT280 ± 10 U/mL

Hafez et al. [46]Rabbit2HC30–60MaS60 (partial)420ALT
AST
178 ± 34 IU/L
406 ± 86 IU/L

Caraceni et al. [32]RatCDD50–60MaS60 (total)120ALT4000 ± 300 IU/L (1000 ± 50 IU/L)1

Marsman et al. [40]RatCMDD>60MaS40 (total)24 hoursALT
AST
Bili
1500 ± 200 (500 ± 100 IU/L)1  
↑ AST (Data not shown)1  
14 ± 3 (6 ± 1  mol/L)1

Selzner et al. [33]MouseCDD>60Mixed45 or 60 (partial)24 hoursAST16566 ± 5731 (500 ± 3395 IU/L)1

Caraceni et al. [34]RatCDD>60MaS6030 to 24 hoursALT8106 ± 1125 (3872 ± 400 IU/L)1

Hakamada et al. [35]RatCDD>60MaS30 or 60 (total)360ALT
Bili
PT
1851.4 ± 66.2 (1861.5 ± 212.6 IU/L)4  
0.67 ± 0.1 (0.14 ± 0.02)1  
21.0 ± 0.9 (18.4 ± 1.3)4

Rolo et al. [47]RatCDD>60Mixed90 (partial)12 hoursALT
AST
1820 ± 5 (1450 ± 20 IU/L)1  
1790 ± 100 (1190 ± 100 IU/L)1

Takahashi et al. [37]CanineHFD7–99MaS6024 hoursALT1400 ± 50 (600 ± 50 IU/L)1

ALT: alanine aminotransferase; AST: aspartate aminotransferase; Bili: bilirubin; CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; D-C: dextrose with cholesterol; HC: high cholesterol; HFD: high-fat diet; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; PT: prothrombin time.
P < 0.05 versus lean livers; no lean group in study; no significant difference between steatotic and lean livers; ALT and PT were significantly higher in steatotic livers compared to lean livers after 30 minutes of ischemia but not after 60 minutes of ischemia.