The Impact of Hepatic Steatosis on Hepatic Ischemia-Reperfusion Injury in Experimental Studies: A Systematic Review

Table 4

Survival outcome in experimental models of orthotopic liver transplantation and hepatic steatosis.

Author	Animal	Steatosis model	% steatosis	Type of steatosis	Duration of ischemia (mins)	Perfusate	Duration of reperfusion	Survival of recipients of steatotic livers (lean livers)
Astarcioglu et al. [48]	Rat	CDD	30–60	MaS	60 or 540	ns	7 days	87.5 and 0 (100 and 100%)^1
Hayashi et al. [49]	Rat	CDD	30–60	MaS	60 to 540	UW	Up to 7 days	0 (75%)^1
Cheng et al. [50]	Rat^2	CMDD	<30 to; >30	Mixed	33–39	UW	ns	33.3% (100%)^1,3
He et al. [31]	Mouse	HFD	50–60	MaS	180	Saline	7 days	0 (40%)^1
Schmeding et al. [51]	Rat^2	CDD	>50	MaS	720	UW	Up to 7 days	68%
Berthiaume et al. [52]	Rat	CMDD	>60	MaS	720	UW	Up to 7 days	0 (85%)^1
Morioka et al. [53]	Rat	HFD	40–50	Mixed	120	HTK	7 days	↓ Survival^4

CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; HFD: high-fat diet; HTK: histidine-tryptophan-ketoglutarate solution; MaS: macrovesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; ns: not stated; Saline: normal saline solution; UW: University of Wisconsin solution.
1P < 0.05 versus lean livers; 2no lean group in study; 3decreased survival in >60% macrovesicular steatosis compared to <60% macrovesicular, >60% microvesicular steatosis or >60% mixed steatosis; 4decreased survival in recipients of 30 and 70% steatotic liver volume compared to recipients of volume matched lean livers.