The Pyridoxal 5′-Phosphate (PLP)-Dependent Enzyme Serine Palmitoyltransferase (SPT): Effects of the Small Subunits and Insights from Bacterial Mimics of Human hLCB2a HSAN1 Mutations

Table 2

Kinetic parameters of wild-type Sp SPT and mutants.

Enzyme	𝑘cat×10^−2; (s^−1)	𝐾^L-ser𝑚; (mM)	𝐾^PCoA𝑚; (𝜇M)	𝑘cat/𝐾^L-ser𝑚; (M^−1 s^−1)	𝑘cat/𝐾^PCoA𝑚; (M^−1 s^−1)	𝐾^L-ser𝑑; (mM)
SPT WT	114±2.0	1.6±0.09	35.6±2.0	712	32022	1.1±0.1
SPT V246M	44±1.2	2.5±0.2	128.0±10.0	176	3437	1.5±0.1
SPT G385F	41±2.0	3.0±0.5	52.2±8.0	136	7854	4.7±0.1
SPT G268V	—	—	—	—	—	—