Demethylation of Cancer/Testis Antigens and CpG ODN Stimulation Enhance Dendritic Cell and Cytotoxic T Lymphocyte Function in a Mouse Mammary Model

Figure 2

The effect of P1A-specific peptide and CpG ODN on DC maturation. (a) DCs positive for CD80, CD86, and CD11c by flow cytometry. (b) ELISA measurements of IL-6 and TNF-α release by cultured DCs. IL-4 and GM-CSF, P1A-specific peptide, CpG ODN, or P1A-specific peptide + CpG ODN were added to DCs for 96 hour. Values are the means ± SD (𝑛=3) of three individual experiments. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 versus control; ^#𝑃<0.05 and ^##𝑃<0.01 versus group treated with P1A-specific peptide or CpG ODN.

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