Role of the eNOS-NO System in Regulating the Antiproteinuric Effects of VEGF Receptor 2 Inhibition in Diabetes

<table>eNOS expression, activity, and urinary excretion of nitric oxide metabolites in nondiabetic (control) and diabetic Ren-2 rats at 24 days. (a) eNOS mRNA determined by real-time PCR. (b) Immunoblotting kidney homogenates from control and diabetic Ren-2 rats for total eNOS protein and eNOS Ser1177 phosphorylation (p-eNOS), indicative of enzyme activation. (c) Urinary nitrate/nitrite. ((d) and (e)) Immunohistochemistry for eNOS in kidney sections from control (d) and diabetic (e) Ren-2 rats. Original magnification ×160. AU: arbitrary units.  <svg height="13.95" id="M17" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.324997 13.95" width="66.324997" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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BioMed Research International

fig2

Figure 2

Figure 2: Role of the eNOS-NO System in Regulating the Antiproteinuric Effects of VEGF Receptor 2 Inhibition in Diabetes 