Role of the eNOS-NO System in Regulating the Antiproteinuric Effects of VEGF Receptor 2 Inhibition in Diabetes

Figure 3

Histological changes in diabetic Ren-2 rats treated with vehicle or vandetanib for 24 days or nondiabetic Ren-2 rats treated with vandetanib for 14 days and then monitored for further 10 days. ((a)–(d)) PAS-stained kidney sections from nondiabetic Ren-2 ((a) and (c)) and diabetic Ren-2 ((b) and (d)) rats treated with vehicle ((a) and (b)) or vandetanib ((c) and (d)). Original magnification ×400. (e) Glomerulosclerosis index. ((f)–(i)) ED-1 immunolabeling in kidney sections from nondiabetic Ren-2 ((f) and (h)) and diabetic Ren-2 ((g) and (i)) rats treated with vehicle ((f) and (g)) or vandetanib ((h) and (i)). Original magnification ×160. (j) Quantitation of cortical ED-1 immunostaining. AU: arbitrary units. ^∗𝑃<0.05 versus nondiabetic Ren-2 + vehicle ^†𝑃<0.05 versus diabetic Ren-2 + vehicle.